Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism in <i>Synechocystis</i> sp. PCC 6803.

ABSTRACT: Cyanobacteria such as Synechocystis sp. PCC 6803 are promising chassis for sustainable bioproduction. During nitrogen starvation, Synechocystis redirects fixed carbon from biomass growth toward glycogen accumulation as a carbon and energy reserve. Inhibiting glycogen synthesis results in the excretion of excess carbon as organic acids, predominantly pyruvate and 2-oxoglutarate. Efficiently rerouting this carbon toward the formation of value-added products such as the plastic alternative polyhydroxybutyrate requires a deeper understanding of carbon partitioning and overflow metabolism. To investigate this, we quantified intra- and extracellular metabolites in Synechocystis wild-type and mutant strains with altered glycogen metabolism (Δpgm, ΔglgC, ΔglgA

SUBMITTER: Alford JT 

PROVIDER: S-EPMC12735552 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature