Development of a TaqMan-Based Duplex Real-Time Quantitative RT-PCR for Detection and Differentiation of Muscovy Duck Reovirus and Novel Duck Reovirus.

ABSTRACT: Muscovy duck reovirus (MDRV) and novel duck reovirus (NDRV) are major pathogens in duck breeding, leading to substantial economic losses in the waterfowl industry. This study aimed to develop a precise detection and differentiation method for both viruses simultaneously. Specific primers and probes targeting the S3 gene were designed, and a duplex TaqMan-based real-time RT-PCR assay was established following optimization of reaction conditions. The assay demonstrated high amplification efficiency (100.1-106.7%), strong linear correlation (R² > 0.999), and low limits of detection (13-25 copies/µL). Intra- and inter-assay coefficients of variation were below 1.5%, confirming excellent repeatability and stability. Applied to 122 clinical duck tissue samples, the assay detected MDRV

SUBMITTER: Liu L 

PROVIDER: S-EPMC12735753 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature