Extraction Behavior and Quantitative Profiling of Prenylated Flavonoids from Hops (<i>Humulus lupulus</i> L.) Under Varying Solvent Polarity, Temperature, and Cryogenic Pretreatment.

ABSTRACT: Prenylated flavonoids from hops (Humulus lupulus L.) represent a distinctive class of bioactive compounds with notable antioxidant and health-promoting properties. This study investigated the extraction behavior and quantitative profiles of three major prenylflavonoids-xanthohumol (XN), isoxanthohumol (IXN), and 8-prenylnaringenin (8-PN)-under varying solvent polarity (ethanol vs. methanol), extraction temperature (50-200 °C), and sample pretreatment (mechanical vs. cryogenic homogenization). Extractions were performed using accelerated solvent extraction (ASE), and compounds were quantified by HPLC-DAD. Ethanol exhibited higher extraction efficiency than methanol, while cryogenic pretreatment markedly enhanced the release of all target analytes. The maximum recovery was achieved at

SUBMITTER: Haring N 

PROVIDER: S-EPMC12735775 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature