A <i>Begomovirus solanumdelhiense</i> Vector for Virus-Induced Gene Silencing in Melon.

ABSTRACT: In this study, the insert length, location within the coat protein-encoding gene, and sequence orientation of the target fragment were optimized to construct an efficient virus-induced gene silencing (VIGS) system in melon using a Begomovirus solanumdelhiense vector. Existing systems are mostly RNA viruses, requiring in vitro synthesis of viral strands that are prone to degradation, although they exhibit high infectivity and stability in cucurbit hosts and ease of manipulation. This vector was selected for its more stable genome structure and these advantages. The melon phytoene desaturase (CmPDS), a key gene of carotenoid biosynthesis, was selected as a reporter gene to evaluate the effects of the VIGS system. Our results revealed that the melon leaves in all the VIGS treatments ex

SUBMITTER: Han Y 

PROVIDER: S-EPMC12735790 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature