Identification and Characterization of Three Novel B-Cell Epitopes in African Swine Fever Virus p22 Protein.

ABSTRACT: African swine fever virus (ASFV) is a highly contagious pathogen causing African swine fever in wild boars, warthogs and domestic pigs. The disease leads tosubstantial economic losses to the global pork industry and poses a grave threat to biodiversity. The early-encoded structural protein p22, owing to its immunodominant characteristics and high conservation across most genotypes, represents a promising diagnostic target and subunit vaccine candidate. In this study, the soluble extracellular domain of p22 protein (aa 30-177) was successfully expressed and purified, yielding 1.220 g/L. Eleven strains of monoclonal antibodies against p22 were generated, with four selected for B-cell epitope screening. Bioinformatic prediction-guided design was employed to generate overlapping truncations an

SUBMITTER: Li Z 

PROVIDER: S-EPMC12735792 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature