A Novel, Broad-Spectrum, Virulent Bacteriophage Targeting <i>Yersinia pestis</i> Isolated from the Soil of Wild Rodent Nests in Yunnan Province, China.

ABSTRACT: As promising biological tools, bacteriophages offer broad potential applications in disease diagnosis, treatment, and food safety. This study is the first to isolate a novel bacteriophage, designated vB_YpP_JC53 (abbreviated JC53), from the soil of wild rodent nests in plague-endemic areas of Yunnan Province. This bacteriophage is a T7-like phage that has the broadest host range among all T7-like phages discovered to date and remains stable under varying temperature and pH conditions. Comparative genomic analysis through NCBI revealed that the nucleotide sequence of phage JC53 shares 94.98% homology (95% coverage) with phage PSTCR2, a member of the Solymavirus genus, while exhibiting substantially lower similarity to known Yersinia phages. Further phylogenetic and collinearity analy

SUBMITTER: Long Y 

PROVIDER: S-EPMC12735813 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature