Engineering Dual-Input Glucose- and Temperature-Sensitive Lysis Circuits in <i>Corynebacterium glutamicum</i> for Efficient Intracellular Product Recovery.

ABSTRACT: Corynebacterium glutamicum is a versatile microbial cell factory, but efficient recovery of intracellular macromolecules remains a major challenge. In this study, we engineered environmentally controllable lysis systems to enable programmable product release. A glucose-responsive module, combining the cg3195 promoter with phage-derived holin-endolysin genes (clg51-50), triggered lysis when extracellular glucose dropped below 0.19-0.36 g/L. A separate temperature-inducible system employing the cI857-CJ1OX2 module activated lysis at 42 °C. These modules were further integrated into a dual-input AND-gate circuit, enhancing regulatory precision and suppressing premature lysis, with additional operator copies allowing temporal tuning of induction. Functional v

SUBMITTER: Ye Z 

PROVIDER: S-EPMC12735821 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature