Synthesis and In Situ Application of a New Fluorescent Probe for Visual Detection of Copper(II) in Plant Roots.

ABSTRACT: A new rhodamine-based fluorescent probe (RDC, rhodamine-based derivative) was rationally designed and synthesized for the highly selective, sensitive, and quantitative detection of Cu²⁺. The probe demonstrated outstanding specificity toward Cu²⁺, even in the presence of competing metal ions (e.g., Al³⁺, Fe³⁺, Cr³⁺, Na⁺, and K⁺), exhibiting negligible interference and confirming its robust anti-interference capability. A spectroscopic analysis revealed that Cu²⁺ induced spirocyclic ring cleavage, resulting in a colorless-to-pink colorimetric transition and enhancement of the yellow-green fluorescence at 590 nm. Upon addition of Cu²⁺, the fluorescence spectrum showed a linear response in the co

SUBMITTER: Hu D 

PROVIDER: S-EPMC12735961 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature