Coixol and Sinigrin from <i>Coix lacryma-jobi</i> L. and <i>Raphanus sativus</i> L. Promote Fat Browning in 3T3-L1 Adipocytes.

ABSTRACT: Background/Objectives: Obesity, a metabolic disorder resulting from an energy imbalance, often leads to excess fat and related diseases. Browning of white adipose tissue, which increases energy expenditure, is a promising anti-obesity strategy. Herbal medicines are considered safer than conventional drugs, but their fat browning mechanisms remain unclear. Therefore, this study aims to examine the effects of Coix lacryma-jobi L. and Raphanus sativus L., alongside their active compounds, coixol and sinigrin. Methods: Cytotoxicity in 3T3-L1 cells was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid accumulation was quantified by the Oil Red O (ORO) staining. Immunofluorescence staining was employed to evaluate mitoc

SUBMITTER: Choi SM 

PROVIDER: S-EPMC12736028 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature