Genomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells.

ABSTRACT:

Background

Transcription by RNA polymerase II is regulated at many steps including initiation, promoter release, elongation and termination. Accumulation of RNA polymerase II at particular locations across genes can be indicative of sites of regulation. RNA polymerase II is thought to accumulate at the promoter and at sites of co-transcriptional alternative splicing where the rate of RNA synthesis slows.

Results

To further understand transcriptional regulation at a global level, we determined the distribution of RNA polymerase II within regions of the human genome designated by the ENCODE project. Hypophosphorylated RNA polymerase II localizes almost exclusively to 5' ends of genes. On the other hand, localization of total RNA polymerase II reveals a variety of distinct land

SUBMITTER: Brodsky AS 

PROVIDER: S-EPMC1273631 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature