The molecular portrait of in vitro growth by meta-analysis of gene-expression profiles.

ABSTRACT:

Background

Cell lines as model systems of tumors and tissues are essential in molecular biology, although they only approximate the properties of in vivo cells in tissues. Cell lines have been selected under in vitro conditions for a long period of time, affecting many specific cellular pathways and processes.

Results

To identify the transcriptional changes caused by long term in vitro selection, we performed a gene-expression meta-analysis and compared 60 tumor cell lines (of nine tissue origins) to 135 human tissue and 176 tumor tissue samples. Using significance analysis of microarrays we demonstrated that cell lines showed statistically significant differential expression of approximately 30% of the approximately 7,000 genes investigated compared to the tissues. Most of

SUBMITTER: Sandberg R 

PROVIDER: S-EPMC1273632 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature