Disruption of the Nitric Oxide Reductase Operon via <i>norD</i> Deletion Does Not Affect <i>Brucella abortus</i> 2308W Virulence.

ABSTRACT: Brucella are intracellular pathogens that use flexible respiratory strategies to adapt to oxygen-limited conditions. The nor operon encodes components of nitric oxide reductase (Nor), which are involved in denitrification and nitric oxide (NO) detoxification. In this study, the role of the norD gene in nitrate-dependent respiration, resistance to nitrosative stress, and intracellular persistence in B. abortus was evaluated. A non-polar ΔnorD mutant was generated in strain 2308W and its survival and growth under aerobic and anaerobic conditions, with and without nitrate, as well as its tolerance to NO donors, were analyzed. In addition, its behavior was evaluated in activated and non-activated murine RAW264.7 and human THP-1 macrophages and in a murine inf

SUBMITTER: Rasheed F 

PROVIDER: S-EPMC12736344 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature