Milk Powder Fortified with Folic Acid and Colostrum Basic Protein Promotes Linear Growth and Improves Bone Microarchitecture in Juvenile Mice Without Adverse Metabolic Effects.

ABSTRACT: Background: The juvenile-pubertal period is a critical window for linear growth and bone mass accumulation. This study investigated the joint effects of folic acid (FA) and colostrum basic protein (CBP)-fortified milk powder on growth, bone health, and metabolic safety in juvenile mice. Methods: Three-week-old C57BL/6J mice (n = 120) were acclimatized for 1 week and then randomly assigned to three isocaloric diet groups for an 8-week intervention starting at 4 weeks of age: Control (AIN-93M), Milk (AIN-93M + FA/CBP-fortified milk powder), and Positive Control (AIN-93G). Body length and weight were measured twice weekly. Bone microarchitecture was assessed by micro-computed tomography, and bone remodeling was evaluated through histology and serum biomarkers. The GH-IGF-1 axis

SUBMITTER: Liu H 

PROVIDER: S-EPMC12736383 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature