Pretreatment with Silver Thiosulfate Increases the Auxin-Inductive Effect for Rooting Mature Chestnut Shoots.

ABSTRACT: Chestnut is a highly valuable species at both the ecological and economic levels, but vegetative propagation protocols have not been optimized for mature material due to its recalcitrant behaviour, thus limiting potential biotechnological applications. In this study, we focused on the formation of adventitious roots in mature chestnut microshoots, which show low rooting responses. Silver thiosulfate, an ethylene-signalling inhibitor, was applied as a pretreatment before auxin induction to study the role of ethylene in this developmental process. Rooting rate was significantly improved in response to the pretreatment, suggesting that ethylene negatively influences the induction of adventitious roots in mature shoots. Transcriptomics and real-time PCR analyses suggest that the improvement in

SUBMITTER: Castro-Camba R 

PROVIDER: S-EPMC12736431 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature