Improving the Culture of Human Skin Explants for Use in Preclinical Testing of Wound Healing Treatments.

ABSTRACT: Background: Cultured human skin explants provide preclinical models to investigate drug delivery and the efficacy of topical treatments for wound healing. However, different culture conditions may affect cell viability, proliferation, and even wound healing. Since animal-derived supplements can influence the investigation of human physiological responses, this study evaluated the effects of non-animal supplements on the ex vivo wound healing process to improve the use of this model for preclinical drug efficacy tests. Methods: In in vitro scratch assays using HaCaT cells and fibroblasts, for media supplemented with normal human serum (NHS), oxygen carriers (OCs) had a positive impact on cell migration, supporting the further evaluation in ex vivo skin culture models. Human sk

SUBMITTER: Guo X 

PROVIDER: S-EPMC12736455 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature