Peas in Rouge: Tyrosine Supplementation Enhances RUBY Reporter Visibility in <i>Pisum sativum</i>.

ABSTRACT: Genome modification of legumes, peas in particular, is accompanied by significant challenges. Establishing a reliable reporter system to identify tissue that expresses foreign DNA may help to optimize and develop transformation protocols for these species. The RUBY system, based on the synthesis of red betalain from tyrosine, offers a convenient solution for monitoring the efficiency of transgene introduction. To evaluate the effectiveness of RUBY application in pea tissue culture, we combined agrobacterial transformation with an in vitro cultivation system, inducing callus development. Transformed explants demonstrated RUBY pigmentation, but it disappeared during cultivation. We hypothesized that this issue is caused by tyrosine depletion. To check this suggestion, we tested whether tyros

SUBMITTER: Simonova V 

PROVIDER: S-EPMC12736525 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature