Electrochemical Immunosensor Based on CS@AuNPs/ZIF-8/rGO Composite for Detecting CA15-3 in Human Serum.

ABSTRACT: An electrochemical immunosensor was fabricated to identify CA15-3, a biomarker for breast cancer (BC). A composite sensor substrate made of "zeolitic imidazolate framework-8" (ZIF-8) and "reduced graphene oxide" (rGO) was chosen and its conductivity was further improved by the addition of chitosan (CS)-doped gold nanoparticles (AuNPs). The CS@AuNPs are able to conjugate with antibodies via the strong Au-S interaction, which offers multiple active sites for antibody immobilization and enhances the sensor performance. This immunosensor is capable of ultrasensitive detection of CA15-3 by specific antigen-antibody -interactions. In healthy people, normal serum CA15-3 is up to 25 U/mL. Under optimized experimental conditions, the alteration in the signal intensity measured by the sensor was rel

SUBMITTER: Lu Y 

PROVIDER: S-EPMC12736644 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature