ALKBH1L Is an m⁶A Demethylase and Mediates PVY Infection in <i>Nicotiana benthamiana</i> Through m⁶A Modification.

ABSTRACT: N⁶-methyladenosine (m⁶A), the most prevalent internal mRNA modification in eukaryotes, is also present in plants and is known to influence plant-virus interactions. However, its specific role in regulating Potato virus Y (PVY; Potyvirus yituberosi) infection, a major pathogen of potatoes, remains unclear. This study identified 16 potential m⁶A regulator genes in Nicotiana benthamiana through homology screening of Arabidopsis thaliana AlkB family members. Based on expression profiles in leaves at various developmental stages and following PVY infection, NbALKBH1L was selected for further analysis. Enzyme assays confirmed its m⁶A demethylase activity. Experiments with NbALKBH1L mutants, using RT-qPCR and m⁶A-I

SUBMITTER: Zhou J 

PROVIDER: S-EPMC12736721 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature