Enhanced <i>Agrobacterium</i>-Mediated Transformation in Chinese Cabbage via Tenoxicam, Phytohormone Optimization, and Visual Reporters.

ABSTRACT: Chinese cabbage (Brassica rapa ssp. pekinensis) is a globally important leafy vegetable, but functional genomics research on its recalcitrance to Agrobacterium-mediated genetic transformation is severely limited. In this study, we demonstrate that both Agrobacterium infection and antibiotic selection significantly inhibit cotyledonary petiole regeneration, representing one principal bottleneck to high-throughput transformation. Infection with different Agrobacterium strains suppressed the regenerated shoot per explant by 30.98-69.16%. Supplying the salicylic acid signaling inhibitor tenoxicam in the seed germination medium raised post-infection regeneration by up to 37.90%. Compared with non-infected controls, the optimal NAA concentration for explant reg

SUBMITTER: Wang S 

PROVIDER: S-EPMC12736769 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature