Comprehensive Insights into Obesity and Type 2 Diabetes from Protein Network, Canonical Pathway, Phosphorylation and Antimicrobial Peptide Signatures of Human Serum.

ABSTRACT:

Background

Obesity is a major risk factor for type 2 diabetes (T2D); however, the molecular links between these conditions are not fully understood.

Methods

We performed an integrative serum proteomics study on samples from 134 individuals (healthy controls, patients with obesity and/or T2D) using both data-independent (DIA) and data-dependent (DDA) liquid chromatography-mass spectrometry approaches, complemented by phosphopeptide enrichment, kinase activity prediction, network and pathway analyses to get more information on the different proteoforms involved in the pathophysiology of the diseases.

Results

We identified 235 serum proteins, including 13 differentially abundant proteins (DAPs) between groups. Both obesity and T2D were characterized by activation of comp

SUBMITTER: Bertalan PM 

PROVIDER: S-EPMC12736859 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature