CRISPR/Cas9-Targeted Gene Editing of Allergenic Profilin-Encoding <i>Lyc e1</i> in Tomato Fruit.

ABSTRACT: Tomato (Solanum lycopersicum L.), one of the most widely consumed horticultural crops worldwide, is also a notable source of food allergens, with a higher prevalence of allergic reactions in pollen-sensitized patients. Profilin, a small actin-binding protein, is a major allergen in tomato fruits encoded by two homologous genes, Lyc e1.01 and Lyc e1.02. Although various strategies have been proposed to reduce allergenicity in foods, no prior study has successfully achieved the complete elimination of profilin proteins in tomato using precise genome editing. In this study, we designed a single-guide RNA targeting a conserved region of both genes and employed the CRISPR/Cas9 system to generate loss-of-function mutations. We first evaluated single-guide RNA editing efficie

SUBMITTER: Yan F 

PROVIDER: S-EPMC12736926 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature