Doubled Haploid Production in <i>Cucurbita pepo</i> L. Through Ovary Culture.

ABSTRACT: Gynogenesis offers a promising route for doubled haploid (DH) production in Cucurbita, yet efficient protocols remain scarce. This study established a reproducible ovary culture system for Cucurbita pepo and evaluated zeatin riboside (ZR) as an alternative cytokinin. Ovaries collected at anthesis and one day before were cultured to screen nine media with different cytokinin-auxin combinations. Subsequently, four optimized ZR-based formulations were evaluated. Both floral stages showed morphogenic activity, but embryo formation occurred almost exclusively in pre-anthesis ovaries. Among ZR treatments, E6.1 (1 mg·L^-1 ZR + 3 mg·L^-1 NAA, 30 g·L^-1 sucrose) achieved the highest embryogenic output (approximately 97 embryos per 100 explants), while hig

SUBMITTER: Garcia-Perez A 

PROVIDER: S-EPMC12737043 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature