A Polycistronic tRNA-amiRNA System Reveals the Antiviral Roles of <i>NbAGO1a/1b/2</i> Against <i>Soybean mosaic virus</i> Infection.

ABSTRACT: RNA interference (RNAi) is a crucial antiviral defense mechanism in plants, where Argonaute (AGO) proteins play a central role. However, the function of AGO proteins in the interaction between Soybean mosaic virus (SMV) and Nicotiana benthamiana remains unclear. In this study, SMV pathogenicity was confirmed using an SMV-GFP infectious clone, with typical symptoms and systemic GFP fluorescence observed 14 days post-inoculation. Real-time quantitative reverse transcription polymerase chain reaction analysis revealed dynamic regulation of multiple NbAGO genes upon infection. Notably, NbAGO1a, NbAGO1b, and NbAGO2 were significantly upregulated and positively correlated with viral accumulation, suggesting their critical roles in antiviral defense. Base

SUBMITTER: Bao W 

PROVIDER: S-EPMC12737065 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature