Intraluminal Vesicles as Transfection Intermediaries.

ABSTRACT: Background: To address hepatotropic body distribution and toxicity, transfection systems based on protein architecture have been proposed. Attenuated anthrax toxin (aATx) has provided the backbone for a first in class transfection system that, in the wild, uses intraluminal vesicles (ILVs) as an intermediary compartment during the translocation of large molecules into the cytosol. Small interfering (si)RNA molecules non-covalently attached to a carrier (LFn-PKR) would not be predicted to be an aATx translocase (protective antigen (PA)) substrate. Previously, siRNA has been shown to be delivered to the cytosol using this system. Methods: Here, the localisation of ³²P-labelled siRNA delivered using aATx was quantified directly and related to siRNA activity. In additi

SUBMITTER: Mahmoud NAM 

PROVIDER: S-EPMC12737133 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature