Functional Analysis of Naturally Integrated <i>Rol</i> Genes in Sweet Potato via CRISPR/Cas9 Genome Editing.

ABSTRACT: Sweet potato (Ipomoea batatas) is a globally important crop and one of a growing number of plants recognized as naturally transgenic, harboring Agrobacterium-derived T-DNA genes whose functions remain largely uncharacterized. In this proof-of-concept study, we applied CRISPR/Cas9 technology to generate targeted knockouts of the Ib-rolB/C and Ib-rolD-like genes located within the sweet potato cellular T-DNA2 (IbT-DNA2) region. Mutations were introduced into sweet potato callus cultures using an optimized genome editing protocol, with most edits consisting of single-nucleotide insertions. Knockout of Ib-rolB/C did not affect callus growth but significantly reduced levels of chlorogenic acid derivatives. Validation in planta using transient exp

SUBMITTER: Shkryl Y 

PROVIDER: S-EPMC12737136 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature