Comparison of the Regulatory Effects of Host Factors on Viral Internal Ribosomal Entry Sites.

ABSTRACT: Host factors play critical roles in viral IRES-mediated translation by modulating the efficiency and specificity of viral protein synthesis. In this study, we used small interfering RNA (siRNA) treatment to silence and plasmid-based expression to overexpress PKD1L3 and USP31. Silencing PKD1L3 and USP31 suppressed IRES activity in FMDV and CSFV RNAs, whereas the overexpression of PKD1L3 did not have a significant effect, and USP31 overexpression resulted in only a modest increase in CSFV-IRES activity. Silencing PKD1L3 significantly reduced EMCV-IRES activity but had no significant effect on HCV- or DENV-IRES activity, and silencing USP31 had no significant effect on the activities of these three IRESs. Notably, the combined overexpression of PKD1L3 and USP31 sig

SUBMITTER: Akhter R 

PROVIDER: S-EPMC12737507 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature