Evaluation of a Cell-Adapted Live Attenuated African Swine Fever Virus Thai-Strain Vaccine Candidate: Highlighting Enhanced Virulence Risk in Co-Infected Pigs.

ABSTRACT:

Background/objectives

African swine fever (ASF) is a devastating disease affecting the swine industry globally. Development of safe and effective vaccines is an urgent need. This study aimed to evaluate, caASFV001-MA52, a cell-adapted ASFV strain derived from serial passaging in MA-104 cells, as a promising live-attenuated vaccine (LAV) candidate against virulent ASFV infection.

Methods

Seven-week-old, crossbred pigs were immunized with caASFV001-MA52 (at a dose of 10⁵ TCID₅₀) and subsequently challenged with a lethal dose of virulent ASFV. Vaccine efficacy was measured through clinical monitoring, immunological and virological analyses, and pathological assessments of tissue protection and viral load reduction. Safety was critically assessed, particula

SUBMITTER: Kaewborisuth C 

PROVIDER: S-EPMC12737558 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature