Relationships Between Metabolism of Cryopreserved Equine Sperm Determined by the Seahorse Analyzer and Sperm Characteristics Measured by Flow Cytometry and Computer-Assisted Analysis of Motility.

ABSTRACT: Cryopreservation of stallion spermatozoa remains a challenge in equine reproduction, as it induces mitochondrial dysfunction that cannot be fully captured by conventional sperm quality assessment and requires further investigation. This study provides a detailed bioenergetic evaluation of cryopreserved stallion sperm using Seahorse XFp technology to measure basal oxygen consumption rate (OCR) and to perform a MitoStress Test. Three ejaculates from 20 Warmblood stallions (licensed by the Holsteiner Verband, Elmshorn, Germany) were analyzed at 15 min intervals from 15 to 120 min after thawing. CASA and multiparametric flow cytometry were used to assess motility, sperm viability, reactive oxygen species (ROS) synthesis, lipid peroxidation (LPO), and DNA fragmentation index (%DFI). Seahorse an

SUBMITTER: Strassner FM 

PROVIDER: S-EPMC12737643 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature