Let-7f-5p Inhibits PRRSV Replication by Regulating Lipid Metabolic Reprogramming in Infected Cells.

ABSTRACT: Lipids provide essential membrane components and energy sources for viral replication, playing multiple roles in viral infection. However, the mutual influence between lipid metabolism and PRRSV proliferation remains unclear. Using transcriptomics, lipidomics, BODIPY staining, and Western blot (WB) analysis, our findings revealed that PRRSV infection significantly altered the abundance of lipid-metabolism-associated genes and lipid metabolites in cells. qRT-PCR confirmed that PRRSV infection dose-dependently upregulated SREBP2 expression (p < 0.01), while BODIPY staining demonstrated a significant increase in intracellular lipid droplets post-infection (p < 0.01). Let-7f-5p significantly reduced lipid droplet accumulation and suppressed PRRSV N protein expression. Notably, 15

SUBMITTER: Jiang D 

PROVIDER: S-EPMC12737658 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature