Host-Adapted Ruminal Microbiota Investigation and Functional Validation of Duolang Sheep-Derived <i>Ligilactobacillus salivarius</i> KS1018.

ABSTRACT: This study characterized the rumen microbiota of Duolang sheep, a Xinjiang local breed, to identify potential probiotics for Hu sheep. 16S rRNA sequencing revealed that Duolang sheep possessed a distinct rumen microbial structure (beta-diversity, p < 0.05), lower diversity (higher Simpson index, p < 0.01), and a higher Firmicutes-to-Bacteroidetes ratio compared to Hu sheep. Subsequently, a Duolang-derived strain, Ligilactobacillus salivarius (L. salivarius) KS1018, was evaluated in a 56-day feeding trial with Hu sheep. Supplementation enhanced systemic immunity (serum IgG and IgM) and antioxidant status (SOD) (p < 0.05). Metabolic profiles were also modified, with serum β-hydroxybutyrate (BHB) level increasing significantly in both medium-dose (p <

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12737677 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature