Pro-fluorescent ethynylthiophene-based <i>o</i>-nitrobenzyl photolabile protecting group for hydroxamic acid synthesis.

ABSTRACT: Photolabile protecting groups (PPGs) that enable real-time monitoring of uncaging processes are highly sought after for tracking product release during and after photolysis. Few PPGs facilitate direct detection of uncaging events through a fluorescence signal, with o-nitrobenzyl (o-NB) PPG derivatives being the only known examples exhibiting pro-fluorescent properties. In this study, we broaden the scope of accessible pro-fluorescent o-NB PPGs for direct monitoring of product release by reporting two new pro-fluorescent, ethynylthiophene-based, and visible light-absorbing o-NB PPGs, referred to as NPETs 1 and 2. UV-Vis spectroscopy confirmed the complete cleavage of hydroxamic acid (HA) derivatives from NPETs 1 and 2, as evidenced by a blue shift and reduced abs

SUBMITTER: Campbell AD 

PROVIDER: S-EPMC12738064 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature