Characterization of a bronchoscopically induced transgenic lung cancer pig model for human translatability.

ABSTRACT: There remains a need for animal models with human translatability in lung cancer (LC) research. Findings in pigs can have a substantial impact owing to their similar anatomy and physiology to humans. Here we present a bronchoscopically induced LC model in Oncopigs carrying inducible KRAS^G12D and TP53^R167H mutations. A total of 12 Oncopigs underwent 29 injections via flexible bronchoscopy: 18 adenovirus-Cre recombinase gene (AdCre) inductions were performed endobronchially (n = 6) and transbronchially (n = 12), and 11 control injections were performed without AdCre. Oncopigs underwent serial chest CT with clinical follow-up for 29 weeks. Lung and organ tissues underwent histopathology, immunohistochemistry and RNA sequencing with comparative analysis to human LC data.

SUBMITTER: Joshi K 

PROVIDER: S-EPMC12738287 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature