Higher diversity and abundance of microRNA-455-5p isoforms confer suppressive role in Wilms' tumor.

ABSTRACT:

Introduction

MicroRNA isoforms (isomiRs) are differentially expressed across tissues, populations, genders, and various disease states, playing significant roles in tumorigenesis and treatment. However, their expression profiles, functions, and clinical relevance in pediatric Wilms' tumour (WT) remain poorly understood.

Methods

Using small RNA-seq data from the TARGET initiative, we profiled isomiRs in WT and adjacent tissues. Bioinformatics tools identified target genes. Key findings were validated in an independent cohort (n=63) using immunohistochemistry (IHC) and multiplex immunofluorescence combined with fluorescence in situ hybridization (mIF-FISH). Associations with histology and survival were analyzed using Kaplan-Meier and Cox regression models.

Results

Our r

SUBMITTER: Chen C 

PROVIDER: S-EPMC12738353 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature