Genome-wide analysis of soybean cinnamyl alcohol dehydrogenase genes identifies GmCAD3 as a positive regulator of <i>Fusarium oxysporum</i> resistance.

ABSTRACT:

Background

Cinnamyl alcohol dehydrogenase (CAD) catalyzes the final step in monolignol biosynthesis and plays critical roles in lignin-mediated plant defense against pathogens. Despite soybean global agricultural importance, systematic characterization of the CAD gene family and its role in disease resistance remains lacking.

Methods

We performed comprehensive genome-wide identification, phylogenetic analysis, and expression profiling of soybean CAD genes. Haplotype diversity was assessed through whole-genome resequencing of 289 Chinese soybean accessions. Population genetic parameters (Fst, π, Tajima's D) were calculated to examine selection signatures. Functional validation was conducted through promoter activity assays, subcellular localization, and transgenic hairy root

SUBMITTER: Zhou R 

PROVIDER: S-EPMC12738375 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature