Newly-engineered angiopoietin-1 as a cell-priming agent for CVD.

ABSTRACT: Human peripheral blood stem cells (PBSC) that have been most frequently used for repair or regeneration in ischemic cardiovascular disease (CVD) showed limitations in their efficacy. We previously reported that angiopoietin-1 (Ang1) is the cell-priming agent to enhance the vasculogenic potential of PBSC. The limitation was the difficulty to produce Ang1 protein with high efficiency. In this study, we engineered Ang1 structure and made FVA3-Ang1 by adding VASP and COMP sequence for stable tetramer formation as well as FLAG sequence for purification in large scale production and a signal peptide derived from influenza A virus (IAV) for better protein expression. FVA3-Ang1 showed stronger effect on endothelial cells than naïve Ang1 or COMP-Ang1 in terms of gene expression of Ang1, Ang2, VEGFA

SUBMITTER: Kang J 

PROVIDER: S-EPMC12738441 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature