A novel accessible in vitro model of proliferative vitreoretinal diseases shows facilitated epithelial mesenchymal transition through aquaporin-1.

ABSTRACT: This study aimed to establish a new in vitro model of proliferative vitreoretinal disease (PVD) using ARPE-19 cells and to evaluate the role of Aquaporin-1 (AQP1) in this model. ARPE-19 cells, stably transfected without or with AQP1, were differentiated in a nicotinamide-enriched medium (N + medium) prior to a 5 day treatment with either N + medium, medium devoid of nicotinamide (N-), or N- medium supplemented with TNF-α and TGF-β (TNT) to induce epithelial-mesenchymal transition (EMT). Human proliferative vitreoretinal disease (PVD) membranes and retinal pigmented cells isolated from ocular globes were collected. RNA sequencing was performed on both ARPE-19 treated cells and human samples. Cell migration assays and immunofluorescence analyses were performed to assess the effects of cell t

SUBMITTER: Datlibagi A 

PROVIDER: S-EPMC12738576 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature