MAASTY: a (dis)ordered copolymer for structural determination of human membrane proteins in native nanodiscs.

ABSTRACT: Amphiphilic copolymers capable of extracting membrane proteins directly from cellular membranes into "native nanodiscs" offer a simplified approach for preparing membrane proteins in lipid nanodiscs compared to approaches that rely on detergent. Copolymer amphiphilicity, length, and composition influence their performance, in addition to the protein itself and the purification conditions used. Here, we report a copolymer composed of methacrylic acid and styrene, which we term MAASTY, leveraging the inherent monomer reactivity ratios to create an anionic copolymer with a statistical distribution of monomers. We show that MAASTY can be used for high-resolution structural determination of a human membrane protein by single particle cryo-electron microscopy, preserving endogenous lipids includ

SUBMITTER: Pugh CF 

PROVIDER: S-EPMC12738716 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature