Unraveling anti-inflammatory metabolic signatures of Glycyrrhiza uralensis and isoliquiritigenin through multiomics.

ABSTRACT: Glycyrrhiza uralensis, a key component of over 70% of traditional herbal medicines (Kampo) in Japan, exhibits diverse pharmacological effects, including immunoregulation, anti-tumor, and antioxidant properties. Despite over 300 identified compounds, the molecular mechanisms remain unclear due to the chemical diversity. Here, we performed a multiomics analysis incorporating untargeted hydrophilic metabolomics, lipidomics, and phosphoproteomics to elucidate the mechanisms distinguishing the G. uralensis extract (GU) from a single bioactive compound, isoliquiritigenin (ILG). Time-course analyses of lipopolysaccharide (LPS)-stimulated RAW264.7 cells under four conditions (control, LPS(+), LPS(+)/ILG(+), and LPS(+)/GU(+)) quantified 182 hydrophilic metabolites, 381 lipids, and 13,211 phosphopep

SUBMITTER: Kiuchi S 

PROVIDER: S-EPMC12738721 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature