PHLDA1 silencing in IMR-32 human neuroblastoma cells results in ABCB1 overexpression, augments chemoresistance and leads to increased growth of tumors.

ABSTRACT: PHLDA1 (pleckstrin homology-like domain, family A, member 1) is a multifunctional protein involved in pathophysiological conditions such as cancer. Our previous research revealed changes in the proteome of PHLDA1-silenced human neuroblastoma IMR-32 cells. One of the proteins that was increased in PHLDA1-silenced cells was the efflux pump ABCB1 (ATP binding cassette subfamily B member 1). In this study, ABCB1 efflux pump upregulation in PHLDA1-silenced IMR-32 cells led to increased resistance of the cells to xenobiotic agents. Experiments revealed that xenograft tumors grown in immunocompromised mice from PHLDA1-silenced cells presented multiple changes in comparison with tumors grown from control cells. Tumors grown from PHLDA1-silenced cells were larger and contained fewer apoptotic cells

SUBMITTER: Kudrycka M 

PROVIDER: S-EPMC12738729 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature