SputOMICs identifies common and distinct markers in cystic fibrosis and chronic obstructive pulmonary disease.

ABSTRACT: Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are muco-obstructive lung diseases. Knowledge of molecular processes has much improved therapeutic options in CF, whereas much less is known for COPD, a disease affecting an increasing number of patients. Here, we report a multilayer workflow integrating microbiome, inflammation and proteome profiling with clinical data to identify disease specific characteristics in sputum. Our proof-of-concept study shows that CF sputum is dominated by Pseudomonas and Staphylococcus, exhibits heightened neutrophilic inflammation, and a severe protease-antiprotease imbalance. In contrast, COPD displays heterogeneous microbiome composition, eosinophilic inflammation, and altered extracellular matrix remodeling. Proteome-based cellular de

SUBMITTER: Frey DL 

PROVIDER: S-EPMC12738762 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature