Structural snapshots of Pseudomonas aeruginosa LptB₂FG and LptB₂FGC reveal insights into lipopolysaccharide recognition and transport.

ABSTRACT: Gram-negative bacteria are intrinsically resistant to many antibiotics because of densely packed lipopolysaccharides (LPS) in the outer leaflet of their outer membrane (OM), which acts as a highly effective barrier towards the spontaneous permeation of toxic molecules, including antibiotics. LPS are extracted from the inner membrane by the ABC transporter LptB₂FGC and translocated across the periplasm via a protein bridge to the OM. While structural studies have elucidated aspects of Lpt function in enterobacteria, little is known about how this system operates in divergent species such as Pseudomonas aeruginosa, a major human pathogen. Here, we report five cryo-electron microscopy structures of P. aeruginosa LptB₂FG and LptB₂FGC, revealing a rigid body mov

SUBMITTER: Fiorentino F 

PROVIDER: S-EPMC12738788 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature