Synthesis of an enhanced nanobiocatalyst system from Aspergillus niger as single green source.

ABSTRACT: The development of sustainable nanobiocatalysts is a focal challenge in green chemistry, requiring robust and eco-friendly production methods. This study introduces a single-source strategy that addresses this challenge. By utilizing the fungus Aspergillus niger as a single biological factory to simultaneously produce a lipase enzyme and biosynthesize the iron oxide nanoparticles (IONPs) that serve as lipase matrix support. This integrated approach ensures a high degree of compatibility between the enzyme and its nanoparticle support, which was confirmed during the immobilization step by an 81.73% yield and a remarkable 97.4% activity retention. The resulting nanobiocatalyst demonstrated a distinct operational stability, broad pH tolerance, and maintained over 80% of its activity after eig

SUBMITTER: Radwan MG 

PROVIDER: S-EPMC12738789 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature