Development of a humanized mouse model of graft-versus-host disease to assess human regulatory T cell function.

ABSTRACT:

Introduction

Regulatory T cells (Treg)-based therapies are increasingly used for treating autoimmune or graft-versus-host (GVHD) disease. Given their low frequency, several approaches aimed at amplifying them or at increasing their immunosuppressive activities are currently investigated. Unfortunately, the impact of these strategies on human Treg function has remained difficult to assess in vivo. Here, we report the development of a novel humanized mouse model of allogeneic and xenogeneic GVHD intended to characterize the immunosuppressive activity of human Treg in vivo.

Methods

In this model, GVHD is induced by injecting CD25-depleted HLA-A2^- peripheral blood mononuclear cells (PBMC) into irradiated NSG-HLA-A2-HHD mice. The CD25⁺ (Treg) f

SUBMITTER: Beguin C 

PROVIDER: S-EPMC12738934 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature