High-Throughput Extracellular Vesicle Isolation Using Plate-Based Size Exclusion Chromatography and Automation.

ABSTRACT: Extracellular vesicles (EVs) are natural, cell-derived nanoparticles released into biofluids, such as plasma, and hold great potential as a new class of biomarkers. However, the utility of analyzing EVs in clinical samples has been hampered by a lack of suitable EV isolation methods that can be performed reproducibly in a scalable manner. The current method of choice for isolating EVs, size exclusion chromatography (SEC), is performed manually one column at a time, and thus does not have the throughput for isolating EVs from clinical samples. In this work, we adapt SEC to a plate-based format to increase its throughput. We show that SEC can be performed using plates containing frits packed with resin, where each well of a 24-well plate can be used for a different sample. By measuring EV ma

SUBMITTER: Gilboa T 

PROVIDER: S-EPMC12739687 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature