MIF/CD74 axis regulates alveolar epithelial type II cell apoptosis, autophagy, and transdifferentiation in bronchopulmonary dysplasia via the TGFβ1/SMAD4 pathway.

ABSTRACT:

Background

Bronchopulmonary dysplasia (BPD) is a prevalent cause of lung injury in premature infants. The migration inhibitory factor (MIF), its receptor CD74, and the TGFβ/Smad signaling cascade play pivotal roles in the regulation of alveolar epithelial type II cells (AECIIs) during lung development. This study investigated whether the MIF/CD74 axis regulates AECIIs by modulating the TGFβ/Smad pathway in BPD.

Materials and methods

A rat model of BPD induced by intraamniotic LPS-injection and postnatal hyperoxia exposure was treated with various interventions and histopathological changes using hematoxylin and eosin staining. In vitro, AECIIs with CD74 or SMAD4 knockdown or overexpression were treated with LPS. Cell proliferation was assessed using BrdU and MTT assays, mRNA

SUBMITTER: Fan M 

PROVIDER: S-EPMC12739859 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature