Volumetric Absorptive Microsampling Combined with Mass Spectrometry to Support Pharmacokinetically-Guided Precision Dosing of Mycophenolate Mofetil in Pediatric Lupus Nephritis Patients.

ABSTRACT:

Background

The immunosuppressant mycophenolate mofetil (MMF) is used off-label in patients with systemic lupus erythematosus (SLE) although the optimum dosing regimen is not well established. This study evaluated the use of volumetric absorptive microsampling (VAMS) for capillary whole blood collected by finger-prick, combined with tandem mass spectrometry and limited timepoint sampling to determine concentrations of mycophenolic acid (MPA) and its glucuronide conjugate, MPA-7-O-glucuronide (MPAG) in SLE patients. This approach permitted pharmacokinetically guided optimized dosing of MPA.

Methods

Blood was collected by finger-prick and venipuncture from patients (n = 10) at trough, 30, and 120 min postdosing with MMF. MPA/MPAG concentrations were assayed from dried VAMS devi

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12739891 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature