Integrated transcriptomic and metabolomic analyses reveal the antibacterial mechanism of isocorydine against <i>Mycobacterium bovis</i>.

ABSTRACT:

Introduction

Mycobacterium bovis, the causative agent of zoonotic tuberculosis, poses a serious threat to public health and agriculture. This study investigates the antibacterial activity and mechanism of action of isocorydine (ICD), a natural alkaloid from Dicranostigma leptopodum (Maxim.) Fedde (DLF), against M. bovis.

Methods

The minimum inhibitory concentration (MIC) of ICD was determined. Phenotypic changes were assessed through assays measuring cell wall/membrane integrity, ion leakage, extracellular pH, total lipid content, and electron microscopy. The global response of M. bovis to sub-inhibitory ICD was elucidated using transcriptomic and metabolomic profiling.

Results

ICD exhibited potent antibacterial activity with a MIC o

SUBMITTER: Wang X 

PROVIDER: S-EPMC12740009 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature