Oocyte Age-Dependent DNA Damage Can Be Reverted by the DNA Repair Competent Karyoplasm of Young Oocytes.

ABSTRACT: Mammalian fully grown oocytes are believed to exhibit a weakened DNA damage response, leading to the accumulation of substantial levels of DNA damage and increased frequency of aneuploidies in an age-dependent manner. These hallmarks of reproductive ageing are generally presumed to be irreversible by rendering the oocyte chromosome complement incompatible with development. To test whether this is indeed true, we performed a series of germinal vesicle (GV) transfers between oocytes from females of late breeding/post-breeding age and oocytes from young animals. Our results show that age-associated DNA damage can be effectively suppressed: introducing the GVs of advanced-maternal-age (AMA) oocytes into DNA repair-competent cytoplasts generated by selective enucleation (SE) of young oocytes ef

SUBMITTER: Dudko N 

PROVIDER: S-EPMC12740095 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature