Identification and validation of biomarkers related to centrosome replication in ulcerative colitis based on bulk transcriptome, single-cell RNA sequencing and experiments.

ABSTRACT:

Background

Ulcerative colitis (UC) is a complex chronic inflammatory disease. Centrosome amplification (CA) has been implicated in UC pathogenesis, but its mechanistic role remains unclear. This study aimed to investigate the relevance of centrosome amplification-related genes (CARGs) in UC progression.

Methods

UC and control samples, along with CARGs, were obtained from public databases. Differential expression analysis identified differentially expressed genes (DEGs) between UC and controls. Candidate genes were selected by intersecting DEGs with CARGs. Biomarker identification employed 11 machine learning algorithms, receiver operating characteristic (ROC) analysis, and expression validation. Functional insights were gained through gene set enrichment analysis (GSEA), imm

SUBMITTER: Yang Z 

PROVIDER: S-EPMC12740759 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature