Cetrorelix promotes cell apoptosis via the PI3K-AKT-FOXO1 pathway in epithelial ovarian cancer.

ABSTRACT:

Introduction

Epithelial ovarian cancer (EOC) has a dismal prognosis, and recent therapeutic advancements have been limited. The aim of our study was to clarify the role and mechanism of cetrorelix in EOC apoptosis and to evaluate the clinical relevance of GnRHR, AKT, and FOXO1 in EOC patients.

Methods

Apoptosis was assessed using flow cytometry, Hoechst staining, and Western blotting. FOXO1, p-AKT and GnRHR knockdown via siRNA was performed to reverse cetrorelix-induced apoptosis. Mechanistic insights were explored using apoptosis gene PCR arrays, qRT-PCR, and Western blotting. In vivo efficacy was tested in a xenograft mouse model. Immunohistochemistry (IHC) was used to assess GnRHR, AKT,p-AKT and FOXO1 expression in EOC tissues, and survival analysis was performed u

SUBMITTER: Zhang N 

PROVIDER: S-EPMC12740856 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature